SH3BP2 antibody (AA 432-481)

Catalog No. ABIN6744458

This Rabbit Polyclonal antibody specifically detects SH3BP2 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Monkey and Cow.

Quick Overview for SH3BP2 antibody (AA 432-481) (ABIN6744458)

Target: SH3BP2 (SH3-Domain Binding Protein 2 (SH3BP2))

Reactivity: Human, Mouse, Rat, Monkey, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH3BP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SH3BP2 Antibody

Binding Specificity: AA 432-481

Brand: IHC-plus™

Specificity: Human SH3BP2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Rabbit, Guinea pig (92%) Bovine (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa432-481 of human SH3BP2 (P78314, NP_003014). Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Mouse, Rat, Opossum (100%), Elephant, Bat, Rabbit, Guinea pig (92%), Galago (85%), Bovine (84%), Zebra finch (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SH3BP2

Target: SH3BP2 (SH3-Domain Binding Protein 2 (SH3BP2))

Alternative Name: SH3BP2

Background: Name/Gene ID: SH3BP2

Synonyms: SH3BP2, Abl-SH3 binding protein 2, 3BP-2, 3BP2, Cherubism, CRPM, RES4-23, SH3 domain-binding protein 2, TNFAIP3 interacting protein 2, CRBM, SH3-domain binding protein 2

Gene ID: 6452

NCBI Accession: NP_003014

UniProt: P78314

Pathways: TCR Signaling