Septin 11 antibody (N-Term)

Catalog No. ABIN6744459

The Rabbit Polyclonal anti-Septin 11 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Septin 11 in samples from Human, Mouse, Rat, Horse, Cow, Guinea Pig, Rabbit, Dog, Monkey and Pig.

Quick Overview for Septin 11 antibody (N-Term) (ABIN6744459)

Target: Septin 11 (SEPT11)

Reactivity: Human, Mouse, Rat, Horse, Cow, Guinea Pig, Rabbit, Dog, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Septin 11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Septin 11 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human SEPT11 / Septin 11

Predicted Reactivity: Percent identity by BLAST analysis: Human, Orangutan, Monkey, Mouse, Rat, Bovine (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human SEPT11 (Q9NVA2, NP_060713). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Septin 11

Target: Septin 11 (SEPT11)

Alternative Name: SEPT11 / Septin 11

Background: Name/Gene ID: SEPT11

Synonyms: SEPT11, Septin 11, Septin-11

Gene ID: 55752

NCBI Accession: NP_060713

UniProt: Q9NVA2