KIF20A antibody (C-Term)

Catalog No. ABIN6744463

Product Details anti-KIF20A Antibody

Target: KIF20A (Kinesin Family Member 20A (KIF20A))

Binding Specificity: C-Term

Reactivity: Human, Rat, Dog, Horse, Pig, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF20A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human KIF20A

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Rabbit, Horse, Pig (100%) Chicken (92%) Rat (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human KIF20A (O95235, NP_005724). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum (100%), Galago, Marmoset, Mouse, Bat, Guinea pig (93%), Turkey, Chicken (92%), Rat (86%), Zebra finch (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for KIF20A

Target: KIF20A (Kinesin Family Member 20A (KIF20A))

Alternative Name: RAB6KIFL / KIF20A (KIF20A Products)

Synonyms: LOC325449 antibody, mt2 antibody, MGC112061 antibody, mklp2 antibody, MGC79056 antibody, rab6kifl antibody, MKLP2 antibody, RAB6KIFL antibody, AA415432 antibody, Rab6kifl antibody, kinesin family member 20A antibody, kinesin family member 20A L homeolog antibody, kinesin-like protein KIF20A antibody, kif20a antibody, kif20a.L antibody, KIF20A antibody, LOC100223319 antibody, LOC100231271 antibody, Kif20a antibody

Background: Name/Gene ID: KIF20A

Synonyms: KIF20A, Kinesin family member 20A, Kinesin-like protein KIF20A, GG10_2, Mitotic kinesin-like protein 2, MKLP2, Rabkinesin-6, RAB6KIFL

Gene ID: 10112

NCBI Accession: NP_005724

UniProt: O95235