NFIC antibody (AA 323-372)

Catalog No. ABIN6744464

This Rabbit Polyclonal antibody specifically detects NFIC in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Pig, Monkey and Bat.

Quick Overview for NFIC antibody (AA 323-372) (ABIN6744464)

Target: NFIC (Nuclear Factor I/C (CCAAT-Binding Transcription Factor) (NFIC))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Pig, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NFIC Antibody

Binding Specificity: AA 323-372

Specificity: Human NFIC

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Guinea pig (100%) Chicken (93%) Xenopus (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa323-372 of human NFIC (Q6FI30, NP_995315). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Bat, Pig, Guinea pig (100%), Zebra finch, Chicken (93%), Opossum (92%), Xenopus (86%), Stickleback (80%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Aliquot to Avoid freeze/thaw cycles after reconstitution

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NFIC

Target: NFIC (Nuclear Factor I/C (CCAAT-Binding Transcription Factor) (NFIC))

Alternative Name: NFI / NFIC

Background: Name/Gene ID: NFIC

Synonyms: NFIC, CTF, CTF5, NF1-C, NF-I/C, NFI, NFI-C, TGGCA-binding protein, NF-I, Nuclear factor 1 C-type, Nuclear factor 1/C, Nuclear factor I/C

Gene ID: 4782

NCBI Accession: NP_995315

UniProt: P08651