CPXCR1 antibody (AA 396-445)

Catalog No. ABIN6744465

Product Details anti-CPXCR1 Antibody

Target: CPXCR1 (CPX Chromosome Region, Candidate 1 (CPXCR1))

Binding Specificity: AA 396-445

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPXCR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human CPXCR1

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Monkey (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa396-445 of human CPXCR1 (Q8N123, NP_149037). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Gibbon, Monkey (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CPXCR1

Target: CPXCR1 (CPX Chromosome Region, Candidate 1 (CPXCR1))

Alternative Name: CPXCR1 / CT77 (CPXCR1 Products)

Background: Name/Gene ID: CPXCR1

Synonyms: CPXCR1, Cancer/testis antigen 77, CT77

Gene ID: 53336

NCBI Accession: NP_149037

UniProt: Q8N123