HIRA antibody (AA 601-650)

Catalog No. ABIN6744564

The Rabbit Polyclonal anti-HIRA antibody is suitable to detect HIRA in samples from Human, Mouse, Dog and Pig. It has been validated for WB.

Quick Overview for HIRA antibody (AA 601-650) (ABIN6744564)

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Reactivity: Human, Mouse, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIRA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HIRA Antibody

Binding Specificity: AA 601-650

Specificity: Mouse HIRA

Predicted Reactivity: Percent identity by BLAST analysis: Mouse (100%) Rabbit (92%) Human, Pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa601-650 of mouse Hira (Q61666, NP_034565). Percent identity by BLAST analysis: Mouse (100%), Gorilla, Monkey, Rabbit, Guinea pig (92%), Human, Gibbon, Galago, Marmoset, Pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HIRA

Target: HIRA (HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))

Alternative Name: HIRA

Background: Name/Gene ID: HIRA

Synonyms: HIRA, DGCR1, HIR, TUPLE1, Protein HIRA

Gene ID: 7290

NCBI Accession: NP_034565

UniProt: P54198

Pathways: Chromatin Binding