HFE2 antibody (AA 35-84)

Catalog No. ABIN6745034

The Rabbit Polyclonal anti-HFE2 antibody (ABIN6745034) specifically detects HFE2 in WB. The antibody is reactive with Human, Mouse, Cow, Rabbit and Monkey samples.

Quick Overview for HFE2 antibody (AA 35-84) (ABIN6745034)

Target: HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))

Reactivity: Human, Mouse, Cow, Rabbit, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HFE2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HFE2 Antibody

Binding Specificity: AA 35-84

Specificity: Human HFE2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine (100%) Rat, Dog, Pig, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human HFE2 (Q6ZVN8, NP_973733). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Elephant, Panda, Bovine, Rabbit (100%), Galago, Rat, Dog, Pig, Opossum, Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HFE2

Target: HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))

Alternative Name: HFE2 / Hemojuvelin

Background: Name/Gene ID: HFE2

Synonyms: HFE2, Haemojuvelin, HFE2A, HJV, Hemochromatosis type 2 protein, Repulsive guidance molecule c, RGM domain family member C, Hemojuvelin, JH, RGMC

Gene ID: 148738

NCBI Accession: NP_973733

UniProt: Q6ZVN8

Pathways: Transition Metal Ion Homeostasis