POLR1E antibody (AA 35-84)

Catalog No. ABIN6745190

The Rabbit Polyclonal anti-POLR1E antibody (ABIN6745190) specifically detects POLR1E in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Hamster, Monkey and Pig samples.

Quick Overview for POLR1E antibody (AA 35-84) (ABIN6745190)

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Hamster, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR1E antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR1E Antibody

Binding Specificity: AA 35-84

Specificity: Human POLR1E / PRAF1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig (100%) Rabbit, Chicken (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human POLR1E (Q9GZS1-2, NP_071935). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Pig, Opossum, Guinea pig (100%), Bat, Rabbit, Chicken (92%), Xenopus (91%), Zebra finch, Platypus (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for POLR1E

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Alternative Name: POLR1E

Background: Name/Gene ID: POLR1E

Synonyms: POLR1E, PRAF1, RNA polymerase I subunit A49, PAF53, RP11-405L18.3

Gene ID: 64425

NCBI Accession: NP_071935

UniProt: Q9GZS1