MED14 antibody (AA 575-624)

Catalog No. ABIN6745238

The Rabbit Polyclonal anti-MED14 antibody has been validated for WB. It is suitable to detect MED14 in samples from Human, Mouse, Rabbit and Monkey.

Quick Overview for MED14 antibody (AA 575-624) (ABIN6745238)

Target: MED14 (Mediator Complex Subunit 14 (MED14))

Reactivity: Human, Mouse, Rabbit, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED14 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MED14 Antibody

Binding Specificity: AA 575-624

Specificity: Human MED14

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa575-624 of human MED14 (O60244, NP_004220). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Rabbit (85%), Mouse (78%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MED14

Target: MED14 (Mediator Complex Subunit 14 (MED14))

Alternative Name: MED14

Background: Name/Gene ID: MED14

Synonyms: MED14, CSRP, CRSP2, CXorf4, CRSP150, DRIP150, EXLM1, Human homolog of yeast RGR1, HRGR1, Mediator complex subunit 14, TRAP170, RGR1 homolog, ARC150, CRSP complex subunit 2, RGR1

Gene ID: 9282

NCBI Accession: NP_004220

UniProt: O60244

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha