SMAD2 antibody (C-Term)

Catalog No. ABIN6745339

This anti-SMAD2 antibody is a Rabbit Polyclonal antibody detecting SMAD2 in WB. Suitable for Human, Mouse, Rat, Cow, Rabbit, Zebrafish (Danio rerio), Chicken, Monkey, Pig, Dog, Horse, Guinea Pig, Bat, Xenopus laevis, Hamster and Drosophila melanogaster.

Quick Overview for SMAD2 antibody (C-Term) (ABIN6745339)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Zebrafish (Danio rerio), Chicken, Monkey, Pig, Dog, Horse, Guinea Pig, Bat, Xenopus laevis, Hamster, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMAD2 Antibody

Binding Specificity: C-Term

Specificity: Human SMAD2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human SMAD2 (Q15796, NP_005892). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Zebrafish, Blood fluke, Drosophila (100%), Turkey, Salmon, Stickleback, Nematode (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name: SMAD2

Background: Name/Gene ID: SMAD2

Synonyms: SMAD2, HMAD-2, HSMAD2, JV18-1, Mad protein homolog smad2, MADR2, MADH2, SMAD 2, MAD homolog 2, Mad-related protein 2, JV18, Mother against DPP homolog 2, Mothers against DPP homolog 2, Sma- and Mad-related protein 2, SMAD family member 2

Gene ID: 4087

NCBI Accession: NP_005892

UniProt: Q15796

Pathways: Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus