Caspase 3 antibody (AA 80-129)

Catalog No. ABIN6745427

The Rabbit Polyclonal anti-Caspase 3 antibody has been validated for WB. It is suitable to detect Caspase 3 in samples from Human, Mouse, Rat, Rabbit, Monkey, Pig, Guinea Pig, Sheep, Hamster and Bat.

Quick Overview for Caspase 3 antibody (AA 80-129) (ABIN6745427)

Target: Caspase 3 (CASP3)

Reactivity: Human, Mouse, Rat, Rabbit, Monkey, Pig, Guinea Pig, Sheep, Hamster, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 3 Antibody

Binding Specificity: AA 80-129

Specificity: Human CASP3 / Caspase 3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Rabbit, Pig (100%) Dog, Horse (92%) Bovine (90%) Mouse, Sheep, Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa80-129 of human CASP3 (P42574, NP_116786). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Rat, Hamster, Elephant, Bat, Rabbit, Pig (100%), Galago, Dog, Cat, Horse (92%), Bovine, Opossum (90%), Mouse, Sheep, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: CASP3 / Caspase 3

Background: Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama

Gene ID: 836

NCBI Accession: NP_116786

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation