ERK2 antibody (AA 148-197)

Catalog No. ABIN6745515

This Rabbit Polyclonal antibody specifically detects ERK2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Monkey, Cow, Rabbit, Horse, Zebrafish (Danio rerio), Chicken, Pig, Guinea Pig, Goat and Xenopus laevis.

Quick Overview for ERK2 antibody (AA 148-197) (ABIN6745515)

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reactivity: Human, Mouse, Rat, Dog, Monkey, Cow, Rabbit, Horse, Zebrafish (Danio rerio), Chicken, Pig, Guinea Pig, Goat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ERK2 Antibody

Binding Specificity: AA 148-197

Specificity: Human MAPK1 / ERK

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa148-197 of human MAPK1 (P28482, NP_620407). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus, Frog, Stickleback, Zebrafish (100%), Drosophila, Beetle (92%), Bat, Salmon (85%), Platypus (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ERK2

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Alternative Name: MAPK1 / ERK2 / p42

Background: Name/Gene ID: MAPK1
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK1, ERK, ERK-2, ERK2, MAP kinase 1, MAPK 2, MAPK2, MAP kinase isoform p42, MAPK 1, p41mapk, PRKM2, MAP kinase 2, p41, p42-MAPK, PRKM1, ERT1, p38, p40, p42MAPK, Protein tyrosine kinase ERK2

Gene ID: 5594

NCBI Accession: NP_620407

UniProt: P28482

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins