NR2E1 antibody (N-Term)

Catalog No. ABIN574489

This Rabbit Polyclonal antibody specifically detects NR2E1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Rabbit, Horse, Cow, Guinea Pig, Zebrafish (Danio rerio), Pig, Bat, Monkey, Chicken, Xenopus laevis, Drosophila melanogaster and Insect.

Quick Overview for NR2E1 antibody (N-Term) (ABIN574489)

Target: NR2E1 (Nuclear Receptor Subfamily 2, Group E, Member 1 (NR2E1))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Horse, Cow, Guinea Pig, Zebrafish (Danio rerio), Pig, Bat, Monkey, Chicken, Xenopus laevis, Drosophila melanogaster, Insect

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR2E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NR2E1 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human NR2E1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Horse, Pig, Chicken, Xenopus, Zebrafish (100%) Rat (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human NR2E1 (Q9Y466, NP_003260). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Sparrow, Chicken, Armadillo, Platypus, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Water flea, Beetle (100%), Turkey (92%), Orangutan, Trout, Salmon, Medaka (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NR2E1

Target: NR2E1 (Nuclear Receptor Subfamily 2, Group E, Member 1 (NR2E1))

Alternative Name: NR2E1 / TLX

Background: Name/Gene ID: NR2E1
Subfamily: NR2 Hepatocyte NF4-like
Family: NHR

Synonyms: NR2E1, HTll, Tailless homolog, Tailless, TLX, TLL, Nuclear receptor TLX, Protein tailless homolog, Tailes-related receptor, XTLL

Gene ID: 7101

NCBI Accession: NP_003260

UniProt: Q9Y466

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance