RAD51 antibody (AA 35-80)

Catalog No. ABIN6745710

This Rabbit Polyclonal antibody specifically detects RAD51 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Cow, Guinea Pig, Monkey, Horse, Pig, Hamster and Xenopus laevis.

Quick Overview for RAD51 antibody (AA 35-80) (ABIN6745710)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Guinea Pig, Monkey, Horse, Pig, Hamster, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD51 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RAD51 Antibody

Binding Specificity: AA 35-80

Brand: IHC-plus™

Specificity: Human RAD51

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Pig (100%) Dog, Chicken, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-80 of human RAD51 (Q06609-2, NP_597994). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Rabbit, Horse, Pig, Guinea pig, Platypus, Xenopus (100%), Dog, Opossum, Turkey, Zebra finch, Chicken, Salmon, Stickleback, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Alternative Name: RAD51 / RECA

Background: Name/Gene ID: RAD51

Synonyms: RAD51, BRCC5, HsT16930, RAD51 homolog A, RAD51 homolog (S. cerevisiae), RecA, E. coli, homolog of, RecA-like protein, Recombination protein A, MRMV2, HRAD51, HsRad51, RAD51A, RECA

Gene ID: 5888

NCBI Accession: NP_597994

UniProt: Q06609

Pathways: DNA Damage Repair