APOB antibody (AA 4031-4080)

Catalog No. ABIN6745726

This anti-APOB antibody is a Rabbit Polyclonal antibody detecting APOB in WB, IHC and IHC (p). Suitable for Human.

Quick Overview for APOB antibody (AA 4031-4080) (ABIN6745726)

Target: APOB (Apolipoprotein B (APOB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-APOB Antibody

Binding Specificity: AA 4031-4080

Brand: IHC-plus™

Specificity: Human APOB

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa4031-4080 of human APOB (P04114, NP_000375). Percent identity by BLAST analysis: Human, Gibbon (100%), Chimpanzee, Gorilla (92%), Monkey (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile dH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for APOB

Target: APOB (Apolipoprotein B (APOB))

Alternative Name: Apolipoprotein B / APOB

Background: Name/Gene ID: APOB

Synonyms: APOB, ApoB-100, Apolipoprotein B-48, ApoB-48, Apolipoprotein B, FLDB, LDLCQ4, Mutant Apo B 100, Apo B-100, Apolipoprotein B-100, Apolipoprotein B48

Gene ID: 338

NCBI Accession: NP_000375

UniProt: P04114

Pathways: Lipid Metabolism