EIF2AK1 antibody (AA 108-157)

Catalog No. ABIN604743

The Rabbit Polyclonal anti-EIF2AK1 antibody (ABIN604743) specifically detects EIF2AK1 in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat, Monkey, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Bat and Pig samples.

Quick Overview for EIF2AK1 antibody (AA 108-157) (ABIN604743)

Target: EIF2AK1 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 1 (EIF2AK1))

Reactivity: Human, Mouse, Rat, Monkey, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Bat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2AK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EIF2AK1 Antibody

Binding Specificity: AA 108-157

Specificity: Human EIF2AK1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Pig, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa108-157 of human EIF2AK1 (Q9BQI3, NP_055228). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Platypus (92%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EIF2AK1

Target: EIF2AK1 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 1 (EIF2AK1))

Alternative Name: EIF2AK1

Background: Name/Gene ID: EIF2AK1
Subfamily: Translational Control Kinase
Family: Protein Kinase

Synonyms: EIF2AK1, Heme-regulated repressor, HRI, KIAA1369, HCR, Heme-controlled repressor, Heme-regulated inhibitor

Gene ID: 27102

NCBI Accession: NP_055228

UniProt: Q9BQI3

Pathways: Hepatitis C