KIT Ligand antibody (AA 180-229)

Catalog No. ABIN604745

The Rabbit Polyclonal anti-KIT Ligand antibody has been validated for WB, IHC and IHC (p). It is suitable to detect KIT Ligand in samples from Human, Mouse, Rat, Pig, Dog, Cow, Goat, Horse, Rabbit, Sheep, Chicken, Hamster and Monkey.

Quick Overview for KIT Ligand antibody (AA 180-229) (ABIN604745)

Target: KIT Ligand (KITLG)

Reactivity: Human, Mouse, Rat, Pig, Dog, Cow, Goat, Horse, Rabbit, Sheep, Chicken, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIT Ligand antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KIT Ligand Antibody

Binding Specificity: AA 180-229

Brand: IHC-plus™

Specificity: Human KITLG

Predicted Reactivity: Percent identity by BLAST analysis: Rat, Horse, Chicken (100%) Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa180-229 of human KITLG (P21583, NP_000890). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Goat, Hamster, Elephant, Dog, Cat, Bovine, Rabbit, Horse, Pig, Opossum, Turkey, Zebra finch, Chicken (100%), Bat, Guinea pig, Platypus (92%), Marmoset (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for KIT Ligand

Target: KIT Ligand (KITLG)

Alternative Name: KITLG / SCF

Background: Name/Gene ID: KITLG

Synonyms: KITLG, C-Kit ligand, KIT ligand, Kitl, KL-1, SHEP7, SF, Steel factor, Stem cell factor, SCF, FPH2, Mast cell growth factor, MGF

Gene ID: 4254

NCBI Accession: NP_000890

UniProt: P21583

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway