Hsc70 antibody (AA 143-192)

Catalog No. ABIN6745743

The Rabbit Polyclonal anti-Hsc70 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Hsc70 in samples from Human, Rat, Mouse, Cow, Chicken, Hamster, Dog, Rabbit, Pig, Guinea Pig, Monkey, Drosophila melanogaster, Xenopus laevis, Zebrafish (Danio rerio), Goat, Horse and Bat.

Quick Overview for Hsc70 antibody (AA 143-192) (ABIN6745743)

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Reactivity: Human, Rat, Mouse, Cow, Chicken, Hamster, Dog, Rabbit, Pig, Guinea Pig, Monkey, Drosophila melanogaster, Xenopus laevis, Zebrafish (Danio rerio), Goat, Horse, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hsc70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Hsc70 Antibody

Binding Specificity: AA 143-192

Brand: IHC-plus™

Specificity: Human HSPA8 / HSC70

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Dog, Rabbit, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa143-192 of human HSPA8 (P11142, NP_006588). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Tamarin, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Trout, Salmon, Stickleback, Medaka, Zebrafish, Drosophila, Slime mold (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Hsc70

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name: HSPA8 / HSC70

Background: Name/Gene ID: HSPA8

Synonyms: HSPA8, Heat shock 70 kDa protein 8, Heat shock 70kd protein 10, Heat shock 70kD protein 8, Heat shock cognate protein 54, HSP71, HSPA10, HSC71, HSP73, LPS-associated protein 1, Heat shock 70  kDa protein 8, NIP71, HSC54, HSC70, LAP1

Gene ID: 3312

NCBI Accession: NP_006588

UniProt: P11142