ATG5 antibody (C-Term)

Catalog No. ABIN6745751

This Rabbit Polyclonal antibody specifically detects ATG5 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Horse, Monkey, Pig, Xenopus laevis and Bat.

Quick Overview for ATG5 antibody (C-Term) (ABIN6745751)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Horse, Monkey, Pig, Xenopus laevis, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATG5 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human ATG5

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Pig, Xenopus (100%) Chicken, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human ATG5 (Q9H1Y0, NP_004840). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Xenopus, Catfish, Beetle (100%), Turkey, Zebra finch, Sparrow, Chicken, Lizard, Salmon, Stickleback, Medaka, Pufferfish, Zebrafish, Drosophila (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: APG5 / ATG5

Background: Name/Gene ID: ATG5

Synonyms: ATG5, APG5, APG5-LIKE, APG5L, Apoptosis specific protein, Apoptosis-specific protein, Autophagy related 5, HAPG5, ASP, Autophagy protein 5

Gene ID: 9474

NCBI Accession: NP_004840

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy