UCHL3 antibody (N-Term)

Catalog No. ABIN6745761

The Rabbit Polyclonal anti-UCHL3 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect UCHL3 in samples from Human, Rat, Mouse, Cow, Horse, Dog, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Pig and Monkey.

Quick Overview for UCHL3 antibody (N-Term) (ABIN6745761)

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Reactivity: Human, Rat, Mouse, Cow, Horse, Dog, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCHL3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UCHL3 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human UCHL3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig, Guinea pig (100%) Chicken (92%) Xenopus, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human UCHL3 (P15374, NP_005993). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Monkey, Mouse, Rat, Panda, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Gorilla, Galago (93%), Chicken, Platypus (92%), Elephant, Lizard (86%), Gibbon, Xenopus, Zebrafish (85%), Grape (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for UCHL3

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Alternative Name: UCHL3

Background: Name/Gene ID: UCHL3
Subfamily: Cysteine C12
Family: Protease

Synonyms: UCHL3, Ubiquitin thiolesterase, Ubiquitin thioesterase L3, UCH-L3

Gene ID: 7347

NCBI Accession: NP_005993

UniProt: P15374

Pathways: Feeding Behaviour, Positive Regulation of fat Cell Differentiation