Amphiregulin antibody (AA 91-140)

Catalog No. ABIN6746007

The Rabbit Polyclonal anti-Amphiregulin antibody has been validated for WB. It is suitable to detect Amphiregulin in samples from Human. There are 2+ publications available.

Quick Overview for Amphiregulin antibody (AA 91-140) (ABIN6746007)

Target: Amphiregulin (AREG)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Amphiregulin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Amphiregulin Antibody

Binding Specificity: AA 91-140

Specificity: Human AREG / Amphiregulin

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Dog, Bovine, Pig (90%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa91-140 of human AREG (P15514, NP_001648). Percent identity by BLAST analysis: Human (100%), Gorilla, Gibbon, Monkey, Marmoset (92%), Dog, Bovine, Horse, Pig (90%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-Amphiregulin antibody (ABIN6746007)

Efendiev, Yudowski, Zwiller, Leibiger, Katz, Berggren, Pedemonte, Leibiger, Bertorello: "Relevance of dopamine signals anchoring dynamin-2 to the plasma membrane during Na+,K+-ATPase endocytosis." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44108-14, (2002) (PubMed).

Chen, Parsons, Brautigan: "Tyrosine phosphorylation of protein phosphatase 2A in response to growth stimulation and v-src transformation of fibroblasts." in: The Journal of biological chemistry, Vol. 269, Issue 11, pp. 7957-62, (1994) (PubMed).

Target Details for Amphiregulin

Target: Amphiregulin (AREG)

Alternative Name: AREG / Amphiregulin

Background: Name/Gene ID: AREG

Synonyms: AREG, Amphiregulin, CRDGF, SDGF

Gene ID: 374

NCBI Accession: NP_001648

UniProt: P15514

Pathways: RTK Signaling, EGFR Signaling Pathway