ARNT antibody (C-Term)

Catalog No. ABIN6746022

The Rabbit Polyclonal anti-ARNT antibody has been validated for WB. It is suitable to detect ARNT in samples from Human, Rat, Cow, Guinea Pig, Pig, Horse, Dog, Monkey and Hamster.

Quick Overview for ARNT antibody (C-Term) (ABIN6746022)

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Reactivity: Human, Rat, Cow, Guinea Pig, Pig, Horse, Dog, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARNT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ARNT Antibody

Binding Specificity: C-Term

Specificity: Rat ARNT

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Horse, Pig, Guinea pig (100%) Mouse, Rat, Rabbit (92%) Xenopus (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of rat Arnt (P41739, NP_036912). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Hamster, Panda, Dog, Bovine, Horse, Pig, Guinea pig (100%), Galago, Mouse, Elephant, Bat, Rabbit, Platypus (92%), Opossum, Xenopus (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ARNT

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Alternative Name: ARNT / HIF-1-Beta

Background: Name/Gene ID: ARNT

Synonyms: ARNT, ARNT protein, BHLHe2, HIF-1beta, HIF1-beta, HIF1BETA, TANGO, HIF-1-beta, HIF1B

Gene ID: 405

NCBI Accession: NP_036912

UniProt: P27540

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect