FMR1 antibody (AA 421-470)

Catalog No. ABIN6746187

This Rabbit Polyclonal antibody specifically detects FMR1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Monkey, Rabbit, Pig, Cow, Bat, Guinea Pig and Chicken and has been mentioned in 2+ publications.

Quick Overview for FMR1 antibody (AA 421-470) (ABIN6746187)

Target: FMR1 (Fragile X Mental Retardation 1 (FMR1))

Reactivity: Human, Mouse, Rat, Dog, Monkey, Rabbit, Pig, Cow, Bat, Guinea Pig, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FMR1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FMR1 Antibody

Binding Specificity: AA 421-470

Specificity: Rat FMR1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig, Guinea pig, Chicken (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa421-470 of rat Fmr1 (Q80WE1, NP_434691). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Pig, Guinea pig, Turkey, Chicken (100%), Platypus, Xenopus (92%), Zebra finch (91%), Opossum (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-FMR1 antibody (ABIN6746187)

Devarajan, De Leon, Talasazan, Schoenfeld, Davidowitz, Burk: "The von Hippel-Lindau gene product inhibits renal cell apoptosis via Bcl-2-dependent pathways." in: The Journal of biological chemistry, Vol. 276, Issue 44, pp. 40599-605, (2001) (PubMed).

Anderson, Teutsch, Dong, Wortis: "An essential role for Bruton's [corrected] tyrosine kinase in the regulation of B-cell apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 20, pp. 10966-71, (1996) (PubMed).

Target Details for FMR1

Target: FMR1 (Fragile X Mental Retardation 1 (FMR1))

Alternative Name: FMR1 / FMRP

Background: Name/Gene ID: FMR1

Synonyms: FMR1, FMRP, POF, Protein FMR-1, Premature ovarian failure 1, Fragile X mental retardation 1, FRAXA, POF1

Gene ID: 2332

NCBI Accession: NP_434691

UniProt: Q06787

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development