GLYT1 antibody (AA 557-606)

Catalog No. ABIN6746199

The Rabbit Polyclonal anti-GLYT1 antibody has been validated for WB. It is suitable to detect GLYT1 in samples from Rat, Human, Mouse, Dog, Guinea Pig, Cow, Horse and Monkey. There are 2+ publications available.

Quick Overview for GLYT1 antibody (AA 557-606) (ABIN6746199)

Target: GLYT1 (Glycine Transporter 1 (GLYT1))

Reactivity: Rat, Human, Mouse, Dog, Guinea Pig, Cow, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLYT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GLYT1 Antibody

Binding Specificity: AA 557-606

Specificity: Mouse SLC6A9 / GLYT1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Guinea pig (100%) Rabbit (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa557-606 of mouse Slc6a9 (P28571-1, NP_032161). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Elephant, Dog, Bovine, Horse, Guinea pig (100%), Galago, Rabbit (92%), Turkey, Chicken (85%), Lizard (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-GLYT1 antibody (ABIN6746199)

Deng, Lin, Wu: "TRAIL-induced apoptosis requires Bax-dependent mitochondrial release of Smac/DIABLO." in: Genes & development, Vol. 16, Issue 1, pp. 33-45, (2002) (PubMed).

Fulda, Meyer, Debatin: "Metabolic inhibitors sensitize for CD95 (APO-1/Fas)-induced apoptosis by down-regulating Fas-associated death domain-like interleukin 1-converting enzyme inhibitory protein expression." in: Cancer research, Vol. 60, Issue 14, pp. 3947-56, (2000) (PubMed).

Target Details for GLYT1

Target: GLYT1 (Glycine Transporter 1 (GLYT1))

Alternative Name: SLC6A9 / GLYT1

Background: Name/Gene ID: SLC6A9
Subfamily: Neurotransmitter:sodium symporter
Family: Transporter

Synonyms: SLC6A9, GLYT1, Glycine transporter 1, GlyT-1, Glycine transporter 1, type 1

Gene ID: 6536

NCBI Accession: NP_032161

UniProt: P48067