PAK4 antibody (AA 23-72)

Catalog No. ABIN6746289

This anti-PAK4 antibody is a Rabbit Polyclonal antibody detecting PAK4 in WB. Suitable for Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse, Zebrafish (Danio rerio), Bat, Hamster and Monkey. This Primary Antibody has been cited in 2+ publications.

Quick Overview for PAK4 antibody (AA 23-72) (ABIN6746289)

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse, Zebrafish (Danio rerio), Bat, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PAK4 Antibody

Binding Specificity: AA 23-72

Specificity: Rat PAK4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa23-72 of rat Pak4 (B5DF62, NP_001099708). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig (100%), Galago, Xenopus (92%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-PAK4 antibody (ABIN6746289)

Marte, Downward: "PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond." in: Trends in biochemical sciences, Vol. 22, Issue 9, pp. 355-8, (1997) (PubMed).

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, (1991) (PubMed).

Target Details for PAK4

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Alternative Name: PAK4

Background: Name/Gene ID: PAK4
Subfamily: PAK (STE20)
Family: Protein Kinase

Synonyms: PAK4, KIAA1142, p21(CDKN1A)-activated kinase 4, PAK-4, p21-activated kinase 4

Gene ID: 10298

NCBI Accession: NP_001099708

UniProt: O96013

Pathways: RTK Signaling