WASF2 antibody (AA 146-195)

Catalog No. ABIN6746329

This Rabbit Polyclonal antibody specifically detects WASF2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Monkey, Pig and Bat and has been mentioned in 4+ publications.

Quick Overview for WASF2 antibody (AA 146-195) (ABIN6746329)

Target: WASF2 (WAS Protein Family, Member 2 (WASF2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Monkey, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WASF2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-WASF2 Antibody

Binding Specificity: AA 146-195

Specificity: Mouse WASF2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa146-195 of mouse Wasf2 (Q8BH43, NP_700472). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Elephant, Turkey, Zebra finch, Xenopus (92%), Stickleback (91%), Dog (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-WASF2 antibody (ABIN6746329)

Ktistaki, Talianidis: "Modulation of hepatic gene expression by hepatocyte nuclear factor 1." in: Science (New York, N.Y.), Vol. 277, Issue 5322, pp. 109-12, (1997) (PubMed).

Kritis, Ktistaki, Barda, Zannis, Talianidis: "An indirect negative autoregulatory mechanism involved in hepatocyte nuclear factor-1 gene expression." in: Nucleic acids research, Vol. 21, Issue 25, pp. 5882-9, (1994) (PubMed).

Kuo, Conley, Hsieh, Francke, Crabtree: "Molecular cloning, functional expression, and chromosomal localization of mouse hepatocyte nuclear factor 1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 24, pp. 9838-42, (1991) (PubMed).

Frain, Swart, Monaci, Nicosia, Stämpfli, Frank, Cortese: "The liver-specific transcription factor LF-B1 contains a highly diverged homeobox DNA binding domain." in: Cell, Vol. 59, Issue 1, pp. 145-57, (1989) (PubMed).

Target Details for WASF2

Target: WASF2 (WAS Protein Family, Member 2 (WASF2))

Alternative Name: SCAR2 / WAVE2

Background: Name/Gene ID: WASF2

Synonyms: WASF2, DJ393P12.2, IMD2, Protein WAVE-2, WASF4, WAVE2, SCAR2, WASP family protein member 2, WASP family protein member 4, WAS protein family, member 2

Gene ID: 10163

NCBI Accession: NP_700472

UniProt: Q9Y6W5

Pathways: RTK Signaling