IRGC antibody (AA 73-122)
(3 references)Quick Overview for IRGC antibody (AA 73-122) (ABIN6746505)
Target
See all IRGC AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 73-122
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Specificity
- Mouse IRGC
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Predicted Reactivity
- Percent identity by BLAST analysis: Mouse (100%).
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Purification
- Immunoaffinity purified
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Immunogen
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Synthetic peptide located between aa73-122 of mouse Irgc1 (Q8C262, NP_950178). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Elephant, Panda, Dog (100%), Galago, Marmoset, Bovine, Bat, Horse, Pig, Guinea pig (92%).
Type of Immunogen: Synthetic peptide
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anti-Immunity-Related GTPase Family, Cinema (IRGC) (C-Term) antibody
IRGC Reactivity: Human, Rat, Mouse WB, ELISA, ICC, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Immunity-Related GTPase Family, Cinema (IRGC) (C-Term) antibodyIRGC Reactivity: Human, Rat, Mouse WB, IF, EIA Host: Rabbit Polyclonal unconjugated
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Application Notes
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Approved: WB (1 μg/mL)
Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody. -
Comment
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Target Species of Antibody: Mouse
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Distilled water
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Concentration
- Lot specific
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Buffer
- Lyophilized from PBS with 2 % sucrose
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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: "Loss of the tight junction proteins occludin and zonula occludens-1 from cerebral vascular endothelium during neutrophil-induced blood-brain barrier breakdown in vivo." in: Neuroscience, Vol. 86, Issue 4, pp. 1245-57, (1998) (PubMed).
: "The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton." in: The Journal of biological chemistry, Vol. 273, Issue 45, pp. 29745-53, (1998) (PubMed).
: "Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues." in: The Journal of cell biology, Vol. 133, Issue 1, pp. 43-7, (1996) (PubMed).
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: "Loss of the tight junction proteins occludin and zonula occludens-1 from cerebral vascular endothelium during neutrophil-induced blood-brain barrier breakdown in vivo." in: Neuroscience, Vol. 86, Issue 4, pp. 1245-57, (1998) (PubMed).
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