PCNP antibody (PEST Proteolytic Signal Containing Nuclear Protein) (C-Term) Primary Antibody
- Binding Specificity
- Bat, Chicken, Cow, Dog, Guinea Pig, Hamster, Horse, Human, Mouse, Pig, Rabbit, Rat
- This PCNP antibody is un-conjugated
- Western Blotting (WB)
- Mouse PCNP
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Chicken (100%) Xenopus (92%).
- Immunoaffinity purified
- Synthetic peptide from C-Terminus of mouse Pcnp (Q6P8I4, NP_001019793). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%), Monkey, Xenopus (92%), Salmon, Stickleback (85%).
Type of Immunogen: Synthetic peptide
- Application Notes
- Approved: WB (1 μg/mL)
Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Target Species of Antibody: Mouse
- For Research Use only
- Distilled water
- Lot specific
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- 4 °C,-20 °C
- Storage Comment
- Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Bolton, Anthony, Perry: "Loss of the tight junction proteins occludin and zonula occludens-1 from cerebral vascular endothelium during neutrophil-induced blood-brain barrier breakdown in vivo." in: Neuroscience, Vol. 86, Issue 4, pp. 1245-57, 1998 (PubMed).
Fanning, Jameson, Jesaitis, Anderson: "The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton." in: The Journal of biological chemistry, Vol. 273, Issue 45, pp. 29745-53, 1998 (PubMed).
Ando-Akatsuka, Saitou, Hirase, Kishi, Sakakibara, Itoh, Yonemura, Furuse, Tsukita: "Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues." in: The Journal of cell biology, Vol. 133, Issue 1, pp. 43-7, 1996 (PubMed).
- Bolton, Anthony, Perry: "Loss of the tight junction proteins occludin and zonula occludens-1 from cerebral vascular endothelium during neutrophil-induced blood-brain barrier breakdown in vivo." in: Neuroscience, Vol. 86, Issue 4, pp. 1245-57, 1998 (PubMed).