Caspase 7 antibody (AA 107-156)

Catalog No. ABIN6746543

The Rabbit Polyclonal anti-Caspase 7 antibody has been validated for WB. It is suitable to detect Caspase 7 in samples from Human, Mouse, Rat, Monkey, Pig, Dog, Horse and Zebrafish (Danio rerio).

Quick Overview for Caspase 7 antibody (AA 107-156) (ABIN6746543)

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Reactivity: Human, Mouse, Rat, Monkey, Pig, Dog, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 7 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 7 Antibody

Binding Specificity: AA 107-156

Specificity: Human CASP7 / Caspase 7

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Pig (100%) Rat, Goat (92%) Chicken (91%) Mouse (85%) Zebrafish (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa107-156 of human CASP7 (P55210, NP_203124). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Rat, Dog, Cat, Horse, Pig, Zebrafish (100%), Galago, Goat, Hamster, Opossum, Chicken (92%), Turkey, Zebra finch (91%), Mouse, Elephant (85%), Bat, Platypus, Seabass, Stickleback (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Caspase 7

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Alternative Name: CASP7 / Caspase 7

Background: Name/Gene ID: CASP7
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP7, Apoptotic protease Mch-3, Caspase 7, Caspase-7, ICE-LAP3, LICE2, MCH3, ICE-like apoptotic protease 3, CASP-7, CMH-1

Gene ID: 840

NCBI Accession: NP_203124

UniProt: P55210

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity