PAR1 antibody (AA 267-316)

Catalog No. ABIN6746560

This Rabbit Polyclonal antibody specifically detects PAR1 in WB. It exhibits reactivity toward Human, Rat, Mouse, Pig and Rabbit and has been mentioned in 3+ publications.

Quick Overview for PAR1 antibody (AA 267-316) (ABIN6746560)

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Reactivity: Human, Rat, Mouse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAR1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PAR1 Antibody

Binding Specificity: AA 267-316

Specificity: Human F2R / Thrombin Receptor

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat (100%) Dog (92%) Pig (85%) Rabbit (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa267-316 of human F2R (P25116, NP_001983). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Mouse, Rat (100%), Gibbon, Baboon, Monkey, Galago, Marmoset, Hamster, Dog, Horse (92%), Pig (85%), Rabbit (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-PAR1 antibody (ABIN6746560)

Gradin, Larsson, Marklund, Gullberg: "Regulation of microtubule dynamics by extracellular signals: cAMP-dependent protein kinase switches off the activity of oncoprotein 18 in intact cells." in: The Journal of cell biology, Vol. 140, Issue 1, pp. 131-41, (1998) (PubMed).

Horwitz, Shen, He, Dittmar, Neef, Chen, Schubart: "The microtubule-destabilizing activity of metablastin (p19) is controlled by phosphorylation." in: The Journal of biological chemistry, Vol. 272, Issue 13, pp. 8129-32, (1997) (PubMed).

Larsson, Marklund, Gradin, Brattsand, Gullberg: "Control of microtubule dynamics by oncoprotein 18: dissection of the regulatory role of multisite phosphorylation during mitosis." in: Molecular and cellular biology, Vol. 17, Issue 9, pp. 5530-9, (1997) (PubMed).

Target Details for PAR1

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Alternative Name: F2R / Thrombin Receptor / PAR1

Background: Name/Gene ID: F2R
Subfamily: Proteinase-activated
Family: GPCR

Synonyms: F2R, CF2R, Coagulation factor II receptor, PAR1, PAR-1, Thrombin receptor, TR, HTR, Protease-activated receptor 1

Gene ID: 2149

NCBI Accession: NP_001983

UniProt: P25116

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus