DNAJC19 antibody (C-Term)

Catalog No. ABIN6746682

This Rabbit Polyclonal antibody specifically detects DNAJC19 in WB. It exhibits reactivity toward Human, Mouse, Rabbit, Cow, Horse, Monkey, Pig and Xenopus laevis and has been mentioned in 3+ publications.

Quick Overview for DNAJC19 antibody (C-Term) (ABIN6746682)

Target: DNAJC19 (DnaJ (Hsp40) Homolog, Subfamily C, Member 19 (DNAJC19))

Reactivity: Human, Mouse, Rabbit, Cow, Horse, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJC19 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DNAJC19 Antibody

Binding Specificity: C-Term

Specificity: Human DNAJC19

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rabbit, Horse, Pig, Xenopus (100%) Chicken, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human DNAJC19 (Q96DA6, NP_660304). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Elephant, Bovine, Rabbit, Horse, Pig, Platypus, Lizard, Xenopus (100%), Guinea pig, Zebra finch, Chicken, Zebrafish (92%), Opossum, Frog, Trout, Salmon (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-DNAJC19 antibody (ABIN6746682)

Straub, Grue, Uhse, Lisby, Knudsen, Tange, Westergaard, Boege: "The RNA-splicing factor PSF/p54 controls DNA-topoisomerase I activity by a direct interaction." in: The Journal of biological chemistry, Vol. 273, Issue 41, pp. 26261-4, (1998) (PubMed).

Basu, Dong, Krainer, Howe: "The intracisternal A-particle proximal enhancer-binding protein activates transcription and is identical to the RNA- and DNA-binding protein p54nrb/NonO." in: Molecular and cellular biology, Vol. 17, Issue 2, pp. 677-86, (1997) (PubMed).

Dong, Horowitz, Kobayashi, Krainer: "Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6." in: Nucleic acids research, Vol. 21, Issue 17, pp. 4085-92, (1993) (PubMed).

Target Details for DNAJC19

Target: DNAJC19 (DnaJ (Hsp40) Homolog, Subfamily C, Member 19 (DNAJC19))

Alternative Name: DNAJC19

Background: Name/Gene ID: DNAJC19

Synonyms: DNAJC19, Homolog of yeast TIM14, PAM18, TIM14, TIMM14

Gene ID: 131118

NCBI Accession: NP_660304

UniProt: Q96DA6

Pathways: SARS-CoV-2 Protein Interactome