CAPN1 antibody (AA 185-234)

Catalog No. ABIN6746925

This Rabbit Polyclonal antibody specifically detects CAPN1 in WB. It exhibits reactivity toward Human, Cow, Pig, Goat, Rabbit, Sheep, Bat, Chicken, Dog, Guinea Pig, Horse, Monkey, Xenopus laevis and Zebrafish (Danio rerio).

Quick Overview for CAPN1 antibody (AA 185-234) (ABIN6746925)

Target: CAPN1 (Calpain 1 (CAPN1))

Reactivity: Human, Cow, Pig, Goat, Rabbit, Sheep, Bat, Chicken, Dog, Guinea Pig, Horse, Monkey, Xenopus laevis, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAPN1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CAPN1 Antibody

Binding Specificity: AA 185-234

Specificity: Human CAPN1 / Calpain 1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Sheep, Dog, Bovine, Goat, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%) Mouse, Rat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa185-234 of human CAPN1 (P07384, NP_005177). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Sheep, Goat, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Xenopus, Trout, Seabass, Salmon, Stickleback, Zebrafish (100%), Mouse, Rat, Elephant, Turkey, Platypus, Lizard (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CAPN1

Target: CAPN1 (Calpain 1 (CAPN1))

Alternative Name: CAPN1 / Calpain 1

Background: Name/Gene ID: CAPN1
Subfamily: Cysteine C2
Family: Protease

Synonyms: CAPN1, Calpain i, Calpain mu-type, Calpain, large polypeptide L1, Calpain-1 catalytic subunit, Calpain 1, Calpain-1 large subunit, CANP, CANP1, Mu CANP, Mu-calpain, Mu-type calpain, MuCANP, Micromolar-calpain, MuCL, CANP 1, CANPL1

Gene ID: 823

NCBI Accession: NP_005177

UniProt: P07384