GPI antibody (C-Term)

Catalog No. ABIN6746935

This Rabbit Polyclonal antibody specifically detects GPI in WB. It exhibits reactivity toward Human, Dog, Monkey, Guinea Pig and Rabbit and has been mentioned in 3+ publications.

Quick Overview for GPI antibody (C-Term) (ABIN6746935)

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Reactivity: Human, Dog, Monkey, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPI antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GPI Antibody

Binding Specificity: C-Term

Specificity: Human GPI

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human GPI (P06744, NP_000166). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset, Rabbit, Guinea pig (100%), Orangutan (92%), Bovine (91%), Panda, Dog, Pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-GPI antibody (ABIN6746935)

Ohashi, Burkart, Flohé, Kolb: "Cutting edge: heat shock protein 60 is a putative endogenous ligand of the toll-like receptor-4 complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 2, pp. 558-61, (2000) (PubMed).

Xanthoudakis, Roy, Rasper, Hennessey, Aubin, Cassady, Tawa, Ruel, Rosen, Nicholson: "Hsp60 accelerates the maturation of pro-caspase-3 by upstream activator proteases during apoptosis." in: The EMBO journal, Vol. 18, Issue 8, pp. 2049-56, (1999) (PubMed).

Venner, Singh, Gupta: "Nucleotide sequences and novel structural features of human and Chinese hamster hsp60 (chaperonin) gene families." in: DNA and cell biology, Vol. 9, Issue 8, pp. 545-52, (1991) (PubMed).

Target Details for GPI

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Alternative Name: GPI

Background: Name/Gene ID: GPI

Synonyms: GPI, AMF, Autocrine motility factor, Glucose phosphate isomerase, Hexose monophosphate isomerase, Hexosephosphate isomerase, Neuroleukin, Oxoisomerase, Phosphoglucose isomerase, Phosphohexomutase, Phosphohexose isomerase, SA36, PGI, SA-36, Glucose-6-phosphate isomerase, GNPI, PHI, Phosphosaccharomutase, Sperm antigen 36, Sperm antigen-36

Gene ID: 2821

NCBI Accession: NP_000166

UniProt: P06744