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Vinculin antibody (AA 873-922)

The Rabbit Polyclonal anti-Vinculin antibody has been validated for WB. It is suitable to detect Vinculin in samples from Human, Mouse, Rat, Rabbit, Chicken, Cow, Horse, Pig, Zebrafish (Danio rerio), Dog, Guinea Pig, Monkey and Bat. There are 2+ publications available.
Catalog No. ABIN6746939

Quick Overview for Vinculin antibody (AA 873-922) (ABIN6746939)

Target

See all Vinculin (VCL) Antibodies
Vinculin (VCL)

Reactivity

  • 128
  • 82
  • 70
  • 27
  • 17
  • 4
  • 4
  • 4
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Human, Mouse, Rat, Rabbit, Chicken, Cow, Horse, Pig, Zebrafish (Danio rerio), Dog, Guinea Pig, Monkey, Bat

Host

  • 99
  • 33
  • 1
Rabbit

Clonality

  • 86
  • 47
Polyclonal

Conjugate

  • 93
  • 7
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Vinculin antibody is un-conjugated

Application

  • 105
  • 48
  • 33
  • 30
  • 27
  • 22
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  • 20
  • 14
  • 13
  • 9
  • 4
  • 3
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 16
    • 9
    • 7
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 873-922

    Specificity

    Human VCL / Vinculin

    Predicted Reactivity

    Percent identity by BLAST analysis: Human, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken, Zebrafish (100%).

    Purification

    Immunoaffinity purified

    Immunogen

    Synthetic peptide located between aa873-922 of human VCL (P18206, NP_003364). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Seabass, Zebrafish (100%), Xenopus (92%).

    Type of Immunogen: Synthetic peptide
  • Application Notes

    Approved: WB (1 μg/mL)

    Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled water

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS with 2 % sucrose

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Mikita, Campbell, Wu, Williamson, Schindler: "Requirements for interleukin-4-induced gene expression and functional characterization of Stat6." in: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5811-20, (1996) (PubMed).

    Hou, Schindler, Henzel, Ho, Brasseur, McKnight: "An interleukin-4-induced transcription factor: IL-4 Stat." in: Science (New York, N.Y.), Vol. 265, Issue 5179, pp. 1701-6, (1994) (PubMed).

  • Target

    Vinculin (VCL)

    Alternative Name

    VCL / Vinculin

    Background

    Name/Gene ID: VCL
    Family: Cytoskeleton

    Synonyms: VCL, CMD1W, CMH15, Metavinculin, Vinculin, MVCL

    Gene ID

    7414

    NCBI Accession

    NP_003364

    UniProt

    P18206

    Pathways

    Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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