ZAP70 antibody (AA 52-101)

Catalog No. ABIN6746955

This anti-ZAP70 antibody is a Rabbit Polyclonal antibody detecting ZAP70 in WB. Suitable for Human, Mouse, Rat, Cow, Monkey, Guinea Pig and Pig.

Quick Overview for ZAP70 antibody (AA 52-101) (ABIN6746955)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human, Mouse, Rat, Cow, Monkey, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ZAP70 Antibody

Binding Specificity: AA 52-101

Specificity: Human ZAP70

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa52-101 of human ZAP70 (P43403, NP_997402). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Mouse (85%), Rat (78%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70

Background: Name/Gene ID: ZAP70
Subfamily: Syk/ZAP70
Family: Protein Kinase

Synonyms: ZAP70, 70 kDa zeta-associated protein, SRK, STCD, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70, TZK, ZAP-70, Zeta-associated protein 70

Gene ID: 7535

NCBI Accession: NP_997402

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway