IFIH1 antibody (AA 612-661)

Catalog No. ABIN6747017

The Rabbit Polyclonal anti-IFIH1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect IFIH1 in samples from Human, Mouse, Rat, Cow, Horse and Pig.

Quick Overview for IFIH1 antibody (AA 612-661) (ABIN6747017)

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Reactivity: Human, Mouse, Rat, Cow, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFIH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IFIH1 Antibody

Binding Specificity: AA 612-661

Brand: IHC-plus™

Specificity: Human IFIH1 / MDA5

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Bovine, Horse (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa612-661 of human IFIH1 (Q9BYX4, NP_071451). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey (100%), Marmoset, Elephant (92%), Bovine, Horse (84%), Panda (83%), Mouse (76%), Rat (75%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for IFIH1

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Alternative Name: IFIH1 / MDA5

Background: Name/Gene ID: IFIH1
Family: DEAD box protein

Synonyms: IFIH1, Helicase with 2 CARD domains, MDA-5, MDA5, Helicard, IDDM19, RLR-2, RH116, CADM-140 autoantigen, Hlcd, RIG-I-like receptor 2

Gene ID: 64135

NCBI Accession: NP_071451

UniProt: Q9BYX4

Pathways: Activation of Innate immune Response