FZD4 antibody (C-Term)

Catalog No. ABIN6747066

This anti-FZD4 antibody is a Rabbit Polyclonal antibody detecting FZD4 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Pig, Cow, Dog, Chicken, Horse, Guinea Pig, Monkey, Bat and Rabbit.

Quick Overview for FZD4 antibody (C-Term) (ABIN6747066)

Target: FZD4 (Frizzled Family Receptor 4 (FZD4))

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog, Chicken, Horse, Guinea Pig, Monkey, Bat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FZD4 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human FZD4 / Frizzled 4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human FZD4 (Q9ULV1, NP_036325). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken (100%), Platypus, Xenopus (92%), Lizard (86%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FZD4

Target: FZD4 (Frizzled Family Receptor 4 (FZD4))

Alternative Name: FZD4 / Frizzled 4

Background: Name/Gene ID: FZD4
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD4, CD344, CD344 antigen, EVR1, Frizzled family receptor 4, Frizzled-4, Fz-4, Fz4, HFz4, Exudative vitreoretinopathy 1, FEVR, FZD4S, FzE4, Frizzled 4, Frizzled homolog 4, WNT receptor frizzled-4

Gene ID: 8322

NCBI Accession: NP_036325

UniProt: Q9ULV1

Pathways: WNT Signaling, Hormone Transport, Sensory Perception of Sound