Occludin antibody (AA 35-84)

Catalog No. ABIN768875

The Rabbit Polyclonal anti-Occludin antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Occludin in samples from Human, Mouse, Rat, Pig, Cow, Dog, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio), Hamster and Monkey.

Quick Overview for Occludin antibody (AA 35-84) (ABIN768875)

Target: Occludin (OCLN)

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio), Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Occludin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Occludin Antibody

Binding Specificity: AA 35-84

Brand: IHC-plus™

Specificity: Human OCLN / Occludin

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Xenopus (92%) Zebrafish (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human OCLN (Q16625, NP_002529). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Opossum, Platypus, Xenopus (92%), Bat (85%), Trout, Zebrafish (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: OCLN / Occludin

Background: Name/Gene ID: OCLN

Synonyms: OCLN, Occludin, BLCPMG

Gene ID: 100506658

NCBI Accession: NP_002529

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C