Transglutaminase 2 antibody (AA 431-480)

Catalog No. ABIN6747111

This Rabbit Polyclonal antibody specifically detects Transglutaminase 2 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Monkey and Rabbit.

Quick Overview for Transglutaminase 2 antibody (AA 431-480) (ABIN6747111)

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reactivity: Human, Mouse, Rat, Monkey, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Transglutaminase 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Transglutaminase 2 Antibody

Binding Specificity: AA 431-480

Specificity: Human TGM2 / Transglutaminase 2

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Guinea pig (92%) Dog, Bovine, Horse, Chicken (85%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa431-480 of human TGM2 (P21980-2, NP_945189). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Rabbit (100%), Hamster, Elephant, Guinea pig (92%), Dog, Bovine, Horse, Pig, Turkey, Zebra finch, Zebrafish (90%), Galago, Bat, Chicken, Seabass, Salmon, Stickleback (85%), Orangutan, Baboon, Xenopus (83%), Medaka (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 0.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Transglutaminase 2

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Alternative Name: TGM2 / Transglutaminase 2

Background: Name/Gene ID: TGM2

Synonyms: TGM2, G-ALPHA-h, GNAH, TG(C), Transglutaminase 2, Transglutaminase C, TG2, TGase H, TGase-2, TGase-H, Transglutaminase H, TGase C, TGC, TTG, C polypeptide, Tgase II, Tissue transglutaminase, Transglutaminase-2

Gene ID: 7052

NCBI Accession: NP_945189

UniProt: P21980

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling