APEH antibody (AA 144-193)

Catalog No. ABIN6747121

The Rabbit Polyclonal anti-APEH antibody has been validated for WB, IHC and IHC (p). It is suitable to detect APEH in samples from Human, Mouse, Rat, Guinea Pig, Rabbit, Cow, Horse, Zebrafish (Danio rerio), Pig, Chicken and Hamster.

Quick Overview for APEH antibody (AA 144-193) (ABIN6747121)

Target: APEH (N-Acylaminoacyl-Peptide Hydrolase (APEH))

Reactivity: Human, Mouse, Rat, Guinea Pig, Rabbit, Cow, Horse, Zebrafish (Danio rerio), Pig, Chicken, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-APEH Antibody

Binding Specificity: AA 144-193

Brand: IHC-plus™

Specificity: Human APEH

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Horse, Pig, Chicken (100%) Dog (92%) Zebrafish (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa144-193 of human APEH (P13798, NP_001631). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Bovine, Rabbit, Horse, Pig, Guinea pig, Turkey, Chicken, Lizard (100%), Monkey, Galago, Elephant, Dog, Bat, Zebra finch (92%), Xenopus, Stickleback (91%), Opossum (85%), Zebrafish (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for APEH

Target: APEH (N-Acylaminoacyl-Peptide Hydrolase (APEH))

Alternative Name: APH / APEH

Background: Name/Gene ID: APEH
Subfamily: Serine S9C
Family: Protease

Synonyms: APEH, Acylpeptide hydrolyase, Acyl-peptide hydrolase, ACPH, DNF15S2, D3F15S2, AARE, Acylaminoacyl-peptidase, OPH, Oxidized protein hydrolase, APH, D3S48E

Gene ID: 327

NCBI Accession: NP_001631

UniProt: P13798