CPN1 antibody (AA 251-300)

Catalog No. ABIN6747138

This anti-CPN1 antibody is a Rabbit Polyclonal antibody detecting CPN1 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Pig and Bat.

Quick Overview for CPN1 antibody (AA 251-300) (ABIN6747138)

Target: CPN1 (Carboxypeptidase N Subunit 1 (CPN1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CPN1 Antibody

Binding Specificity: AA 251-300

Brand: IHC-plus™

Specificity: Human CPN1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Rabbit, Horse, Pig, Guinea pig (100%) Chicken (92%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa251-300 of human CPN1 (P15169, NP_001299). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Marmoset, Opossum, Turkey, Zebra finch, Chicken (92%), Lizard, Zebrafish (84%), Drosophila (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CPN1

Target: CPN1 (Carboxypeptidase N Subunit 1 (CPN1))

Alternative Name: CPN1 / ACBP

Background: Name/Gene ID: CPN1
Subfamily: Metallopeptidase M14
Family: Exopeptidase

Synonyms: CPN1, ACBP, Arginine carboxypeptidase, Anaphylatoxin inactivator, Carboxypeptidase K, Kininase-1, Lysine carboxypeptidase, Kininase I, Serum carboxypeptidase N, SCPN, Plasma carboxypeptidase B, CPN

Gene ID: 1369

NCBI Accession: NP_001299

UniProt: P15169

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, C21-Steroid Hormone Metabolic Process