DENND1B antibody (AA 144-193)

Catalog No. ABIN6747143

The Rabbit Polyclonal anti-DENND1B antibody has been validated for WB, IHC and IHC (p). It is suitable to detect DENND1B in samples from Human, Mouse, Rabbit, Guinea Pig, Horse, Dog, Zebrafish (Danio rerio), Bat, Chicken, Monkey and Pig.

Quick Overview for DENND1B antibody (AA 144-193) (ABIN6747143)

Target: DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

Reactivity: Human, Mouse, Rabbit, Guinea Pig, Horse, Dog, Zebrafish (Danio rerio), Bat, Chicken, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DENND1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DENND1B Antibody

Binding Specificity: AA 144-193

Brand: IHC-plus™

Specificity: Human DENND1B

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Rabbit, Horse, Pig, Guinea pig, Chicken (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa144-193 of human DENND1B (Q6P3S1, NP_659414). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Dog, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Stickleback, Zebrafish (100%), Bovine (92%), Rat, Panda, Lizard, Xenopus, Beetle (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for DENND1B

Target: DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

Alternative Name: DENND1B

Background: Name/Gene ID: DENND1B

Synonyms: DENND1B, C1ORF18, C1orf218, Connecdenn 2, DENN/MADD domain containing 1B, Protein FAM31B, FAM31B, RP11-53I24.2

Gene ID: 163486

NCBI Accession: NP_659414

UniProt: Q6P3S1