DENND1B antibody (N-Term)

Catalog No. ABIN654339

Product Details anti-DENND1B Antibody

Target: DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

Binding Specificity: AA 39-68, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DENND1B antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: M

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This DENND1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the N-terminal region of human DENND1B.

Clone: RB28056

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:500. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for DENND1B

Target: DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

Alternative Name: DENND1B (DENND1B Products)

Synonyms: C1ORF18 antibody, C1orf218 antibody, FAM31B antibody, 4632404N19Rik antibody, 4930467M19Rik antibody, 6820401H01Rik antibody, F730008N07Rik antibody, Fam31b antibody, si:ch211-195i21.1 antibody, DENN domain containing 1B antibody, DENN/MADD domain containing 1B antibody, DENND1B antibody, dennd1b antibody, Dennd1b antibody

Background: Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2, see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).

Molecular Weight: 86552

Gene ID: 163486

NCBI Accession: NP_001182144, NP_001182145, NP_659414

UniProt: Q6P3S1