SQSTM1 antibody (AA 323-372)

Catalog No. ABIN768977

The Rabbit Polyclonal anti-SQSTM1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect SQSTM1 in samples from Human, Mouse, Rat, Cow, Rabbit, Monkey, Sheep, Horse, Pig, Dog, Guinea Pig, Chicken and Bat.

Quick Overview for SQSTM1 antibody (AA 323-372) (ABIN768977)

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Monkey, Sheep, Horse, Pig, Dog, Guinea Pig, Chicken, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SQSTM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SQSTM1 Antibody

Binding Specificity: AA 323-372

Brand: IHC-plus™

Specificity: Human SQSTM1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Sheep, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken (100%) Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa323-372 of human SQSTM1 (Q13501, NP_003891). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%), Xenopus, Salmon, Stickleback, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: p62 / SQSTM1

Background: Name/Gene ID: SQSTM1

Synonyms: SQSTM1, A170, ORCA, OSIL, Paget disease of bone 3, p60, p62, PDB3, Sequestosome-1, Ubiquitin-binding protein p62, Oxidative stress induced like, p62B, ZIP3, Sequestosome 1, EBI3-associated protein p60, EBIAP

Gene ID: 8878

NCBI Accession: NP_003891

UniProt: Q13501

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy