NLRP1 antibody (AA 323-372)

Catalog No. ABIN768983

The Rabbit Polyclonal anti-NLRP1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect NLRP1 in samples from Human, Mouse, Cow, Dog, Horse and Monkey.

Quick Overview for NLRP1 antibody (AA 323-372) (ABIN768983)

Target: NLRP1 (NLR Family, Pyrin Domain Containing 1 (NLRP1))

Reactivity: Human, Mouse, Cow, Dog, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NLRP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NLRP1 Antibody

Binding Specificity: AA 323-372

Brand: IHC-plus™

Specificity: Human NLRP1 / NALP1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse (100%) Rat (92%) Dog (85%) Bovine (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa323-372 of human NLRP1 (Q9C000, NP_001028225). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse (100%), Rat, Opossum (92%), Platypus, Rice (91%), Gorilla, Dog (85%), Galago, Elephant, Bovine, Rabbit, Horse, Pig, Guinea pig (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NLRP1

Target: NLRP1 (NLR Family, Pyrin Domain Containing 1 (NLRP1))

Alternative Name: NALP1 / NLRP1

Background: Name/Gene ID: NLRP1

Synonyms: NLRP1, CLR17.1, CARD7, DEFCAP, DEFCAP-L/S, NAC, SLEV1, VAMAS1, KIAA0926, NALP1, PP1044

Gene ID: 22861

NCBI Accession: NP_001028225

UniProt: Q9C000

Pathways: Positive Regulation of Endopeptidase Activity, Inflammasome