Lactate Dehydrogenase A antibody (N-Term)

Catalog No. ABIN6747164

The Rabbit Polyclonal anti-Lactate Dehydrogenase A antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Lactate Dehydrogenase A in samples from Human, Rabbit, Pig, Cow, Horse, Monkey and Hamster.

Quick Overview for Lactate Dehydrogenase A antibody (N-Term) (ABIN6747164)

Target: Lactate Dehydrogenase A (LDHA)

Reactivity: Human, Rabbit, Pig, Cow, Horse, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lactate Dehydrogenase A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Lactate Dehydrogenase A Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human LDHA

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Guinea pig (92%) Bovine, Rabbit, Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human LDHA (P00338, NP_005557). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Hamster (100%), Galago, Mouse, Rat, Guinea pig (92%), Ferret, Dolphin, Bovine, Rabbit, Horse, Opossum, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Lactate Dehydrogenase A

Target: Lactate Dehydrogenase A (LDHA)

Alternative Name: LDHA / LDH1

Background: Name/Gene ID: LDHA

Synonyms: LDHA, LDH muscle subunit, LDH1, Lactate dehydrogenase M, LDH-M, GSD11, Lactate dehydrogenase A, LDH-A, LDHM, PIG19, Proliferation-inducing gene 19

Gene ID: 3939

NCBI Accession: NP_005557

UniProt: P00338

Pathways: Warburg Effect