PPID antibody (AA 71-120)

Catalog No. ABIN6747179

The Rabbit Polyclonal anti-PPID antibody has been validated for WB, IHC and IHC (p). It is suitable to detect PPID in samples from Human, Mouse, Rat, Rabbit, Dog, Guinea Pig, Cow, Horse, Zebrafish (Danio rerio), Monkey, Pig, Bat, Chicken and Xenopus laevis.

Quick Overview for PPID antibody (AA 71-120) (ABIN6747179)

Target: PPID (Peptidylprolyl Isomerase D (PPID))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Guinea Pig, Cow, Horse, Zebrafish (Danio rerio), Monkey, Pig, Bat, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPID antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PPID Antibody

Binding Specificity: AA 71-120

Brand: IHC-plus™

Specificity: Human PPID / Cyclophilin 40

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig, Chicken, Zebrafish (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa71-120 of human PPID (Q08752, NP_005029). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Trout, Salmon, Zebrafish (100%), Galago, Elephant, Stickleback, Arabidopsis (92%), Drosophila (91%), Blood fluke (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PPID

Target: PPID (Peptidylprolyl Isomerase D (PPID))

Alternative Name: PPID / Cyclophilin D

Background: Name/Gene ID: PPID

Synonyms: PPID, Cyclophilin-40, CYP40, CYP-40, Cyclophilin 40, Cyclophilin-related protein, CYPD, PPIase D, Cyclophilin D, Peptidylprolyl isomerase D, Rotamase D

Gene ID: 5481

NCBI Accession: NP_005029

UniProt: Q08752

Pathways: Nuclear Hormone Receptor Binding