Nestin antibody (AA 1295-1344)

Catalog No. ABIN6747184

This Rabbit Polyclonal antibody specifically detects Nestin in WB, IHC and IHC (p). It exhibits reactivity toward Human, Dog and Horse.

Quick Overview for Nestin antibody (AA 1295-1344) (ABIN6747184)

Target: Nestin (NES)

Reactivity: Human, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Nestin Antibody

Binding Specificity: AA 1295-1344

Brand: IHC-plus™

Specificity: Human NES / Nestin

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa1295-1344 of human NES (P48681, NP_006608). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Monkey (92%), Marmoset, Horse (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

Background: Name/Gene ID: NES

Synonyms: NES, Nestin, Nbla00170

Gene ID: 10763

NCBI Accession: NP_006608

UniProt: P48681