ATG12 antibody (AA 79-128)

Catalog No. ABIN6747201

This anti-ATG12 antibody is a Rabbit Polyclonal antibody detecting ATG12 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Monkey, Chicken, Rabbit, Pig, Bat and Xenopus laevis.

Quick Overview for ATG12 antibody (AA 79-128) (ABIN6747201)

Target: ATG12 (Autophagy Related 12 (ATG12))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Monkey, Chicken, Rabbit, Pig, Bat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATG12 Antibody

Binding Specificity: AA 79-128

Brand: IHC-plus™

Specificity: Rat ATG12

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Guinea pig, Chicken, Xenopus (100%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa79-128 of rat Atg12 (Q2TBJ5, NP_001033584). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Xenopus (100%), Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: APG12 / ATG12

Background: Name/Gene ID: ATG12

Synonyms: ATG12, Autophagy-related protein 12, APG12L, Autophagy related 12, FBR93, APG12, APG12-like, Ubiquitin-like protein ATG12, HAPG12

Gene ID: 9140

NCBI Accession: NP_001033584

UniProt: O94817

Pathways: Autophagy