CD40 Ligand antibody (AA 108-157)

Catalog No. ABIN6748490

This Rabbit Polyclonal antibody specifically detects CD40 Ligand in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Pig, Cow, Monkey, Dog, Sheep, Horse and Bat.

Quick Overview for CD40 Ligand antibody (AA 108-157) (ABIN6748490)

Target: CD40 Ligand (CD40LG)

Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey, Dog, Sheep, Horse, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD40 Ligand Antibody

Binding Specificity: AA 108-157

Brand: IHC-plus™

Specificity: Human CD40LG / CD40L

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Pig (100%) Mouse, Rat (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa108-157 of human CD40LG (P29965, NP_000065). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Sheep, Dog, Bovine, Bat, Horse, Pig (100%), Elephant, Rabbit (92%), Cat (90%), Galago (84%), Mouse, Rat (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40LG / CD40L / CD154

Background: Name/Gene ID: CD40LG
Family: TNF

Synonyms: CD40LG, CD154, CD154 antigen, CD40-L, CD40 ligand, CD40L, gp39, HIGM1, IGM, IMD3, T-BAM, TNFSF5, CD40 antigen ligand, CD54, HCD40L, T-B cell-activating molecule, T-cell antigen Gp39, TNF-related activation protein

Gene ID: 959

NCBI Accession: NP_000065

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints